UJI TOKSISITAS SUBKRONIK EKSTRAK TERPURIFIKASI DAUN PACAR KUKU (Lawsonia inermis L.) TERHADAP HATI TIKUS

Abstract

Pacar Kuku plant (Lawsonia inermis L.) has the main content of active compounds such as flavonoids, glycosides, alkaloids, phenols, essential oils, and glycosides. The most active compounds found in Pacar Kuku leaves are phenols and flavonoids. Pacar Kuku leaves have many pharmacological activities including antimicrobial activity, diabetes mellitus treatment, cytotoxic, antiviral and wound healing effects. The purpose of this study was to determine the subchronic toxicity of purified extract of henna leaves by looking at the histological picture of the liver and symptoms of toxicity in mice. The method of this study was that the experimental animals were divided into 4 groups. The division of treatment groups is as follows: Group 1 as the control group was given Na. CMC 1%, groups 2 to 4 were given purified extract of Pacar Kuku leaves with dose variations of 250 mg / KgBW, 500 mg / KgBW and 1000 mg / KgBW given for 28 days. For 28 days, the animals were observed for toxicity symptoms such as respiratory rate, fatigue, body movement, fur appearance, gait, trembling, saliva production, and urination frequency and histopathological analysis of the heart was performed on the 28th day. Administration of purified extract of Pacar Kuku leaves at doses of 250 mg/kgBW, 500 mg/kgBW and 1000 mg/kgBW did not cause toxicity symptoms but affected the histopathological picture of the liver in mice which was characterized by cell damage in the form of necrosis, fatty degeneration and hydropic degeneration in each treatment group.