STUDI IN VIVO GEL FRAKSI ETIL ASETAT Aloe vera UNTUK PENYEMBUHAN LUKA BAKAR

Abstract

Aedes aegypti is the primary vector of dengue fever, a major public health concern worldwide. This study aimed to evaluate the larvicidal activity of ethanol extract of garlic peel (Allium sativum L.) and to analyze the effect of different extract concentrations on larval mortality. The extract was obtained using the reflux extraction method with 70% ethanol as the solvent. An experimental laboratory study was conducted by exposing third-instar A. aegypti larvae to various concentrations of the extract. Larval mortality was analyzed using probit analysis to determine the LC90 and LT90 values. Mortality observations were carried out at 30, 60, 120, 240, 480, 720, and 1440 minutes (24 hours) after treatment. Statistical analysis revealed significant differences in larval mortality among treatment groups (p < 0.05). LT50 and LT90 values were estimated using probit analysis and should be presented together with their 95% Confidence Intervals (CI) to indicate the precision of the estimates. The results demonstrated that the ethanol extract of garlic peel exhibited larvicidal activity, with larval mortality increasing as the extract concentration increased. The LC90 and LT90 values confirmed the effectiveness of the extract as a larvicidal agent. These findings suggest that garlic peel has the potential to serve as a safe, environmentally friendly, and sustainable plant-based larvicide for the control of Aedes aegypti populations